7WDW
DsyB in complex with SAH and MTHB
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL18U1 |
| Synchrotron site | SSRF |
| Beamline | BL18U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-12-05 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.979 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 88.103, 69.292, 104.340 |
| Unit cell angles | 90.00, 92.19, 90.00 |
Refinement procedure
| Resolution | 37.160 - 2.390 |
| R-factor | 0.191 |
| Rwork | 0.187 |
| R-free | 0.26800 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 7DFD |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.6.4_486) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.490 |
| High resolution limit [Å] | 2.390 | 2.400 |
| Rmerge | 0.094 | 0.457 |
| Number of reflections | 49532 | 5100 |
| <I/σ(I)> | 11.6 | |
| Completeness [%] | 99.7 | 99.7 |
| Redundancy | 3.7 | 3.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 0.2 M NaCl, 0.1 M Tris (pH 8.0) and 20% PEG 4000 |
