7WDS
Crystal structures of MeBglD2 in complex with various saccharides
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL44XU |
| Synchrotron site | SPring-8 |
| Beamline | BL44XU |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2016-05-19 |
| Detector | RAYONIX MX300HE |
| Wavelength(s) | 0.9 |
| Spacegroup name | I 4 3 2 |
| Unit cell lengths | 203.169, 203.169, 203.169 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 41.470 - 1.680 |
| R-factor | 0.16847 |
| Rwork | 0.165 |
| R-free | 0.22864 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5xgz |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.975 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0238) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.710 |
| High resolution limit [Å] | 1.680 | 1.680 |
| Rmerge | 0.098 | 0.745 |
| Number of reflections | 80168 | 3894 |
| <I/σ(I)> | 27.8 | |
| Completeness [%] | 99.9 | |
| Redundancy | 19.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 0.1 M Tris-HCl buffer (pH 8.5), 1.2 M lithium sulfate, 0.01 M nickel chloride |
