7WDN
Crystal structures of MeBglD2 in complex with various saccharides
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL44XU |
| Synchrotron site | SPring-8 |
| Beamline | BL44XU |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-12-08 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.9 |
| Spacegroup name | I 4 3 2 |
| Unit cell lengths | 202.488, 202.488, 202.488 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 30.000 - 1.800 |
| R-factor | 0.16186 |
| Rwork | 0.159 |
| R-free | 0.21005 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5xgz |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.774 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0238) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.830 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.063 | 0.637 |
| Number of reflections | 61903 | 61901 |
| <I/σ(I)> | 25.6 | |
| Completeness [%] | 99.9 | |
| Redundancy | 13 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 0.1 M Tris-HCl buffer (pH 8.5), 1.2 M lithium sulfate, 0.01 M nickel chloride |
