7WDL
Fungal immunomodulatory protein FIP-nha
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U1 |
| Synchrotron site | SSRF |
| Beamline | BL17U1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2019-11-06 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.979183 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 101.256, 119.453, 110.224 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 26.256 - 1.903 |
| R-factor | 0.1774 |
| Rwork | 0.176 |
| R-free | 0.20670 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3f3h |
| Data reduction software | HKL-2000 (v7.21) |
| Data scaling software | HKL-2000 (v7.21) |
| Phasing software | PHASER (2.7.0) |
| Refinement software | PHENIX (1.15.2_3472) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 26.256 | 1.971 |
| High resolution limit [Å] | 1.903 | 1.903 |
| Rmerge | 0.065 | 0.416 |
| Number of reflections | 52430 | 5104 |
| <I/σ(I)> | 25.57 | |
| Completeness [%] | 99.8 | |
| Redundancy | 8.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5 | 293.15 | 4% v/v TassimateTM pH 5.0 14% w/v PEG 3,350 |
