7WCY

Crystal Structure of H-2Kb with Cryptosporidium parvum gp40/15 epitope

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU MICROMAX-007 HF
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2021-11-20
Detector	MAR scanner 300 mm plate
Wavelength(s)	0.97915
Spacegroup name	P 1 21 1
Unit cell lengths	60.920, 84.770, 95.240
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	49.470 - 2.360
Rwork	0.226
R-free	0.25840
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1vac
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	PHASER
Refinement software	REFMAC (5.8.0258)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	49.470	2.450
High resolution limit [Å]	2.360	2.360
Number of reflections	40181	4091
<I/σ(I)>	15.259
Completeness [%]	98.0
Redundancy	2.5
CC(1/2)	0.999	0.826

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	291	12%(w/v) Polyethylene glycol 3350, 0.005M Cobalt(II) chloride hexahydrate; 0.005M Nickel(II) chloride hexahydrate; 0.005M Cadmium chloride hydrate; 0.1M HEPES (PH7.5)