7WBT

Crystal structure of bovine NLRP9

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL32XU
Synchrotron site	SPring-8
Beamline	BL32XU
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-11-19
Detector	DECTRIS EIGER X 9M
Wavelength(s)	1.0000
Spacegroup name	P 21 21 21
Unit cell lengths	102.260, 169.120, 177.840
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	49.140 - 2.750
R-factor	0.2332
Rwork	0.232
R-free	0.26150
Structure solution method	MOLECULAR REPLACEMENT
Data reduction software	XDS
Data scaling software	XDS
Phasing software	MOLREP
Refinement software	PHENIX (1.19.2_4158)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	177.840	2.830
High resolution limit [Å]	2.670	2.670
Number of reflections	88250	13957
<I/σ(I)>	27.9
Completeness [%]	100.0
Redundancy	427.6
CC(1/2)	0.999	0.774

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	6.25-7.50% PEG20000, 100 mM MES pH 6.5