7WBM

Crystal structure of Legionella pneumophila effector protein Lpg0081

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 5C (4A)
Synchrotron site	PAL/PLS
Beamline	5C (4A)
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-11-30
Detector	DECTRIS EIGER X 9M
Wavelength(s)	1.0000
Spacegroup name	C 1 2 1
Unit cell lengths	116.968, 92.747, 109.223
Unit cell angles	90.00, 105.94, 90.00

Refinement procedure

Resolution	35.830 - 2.700
R-factor	0.1568
Rwork	0.154
R-free	0.20250
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	7wbk
RMSD bond length	0.009
RMSD bond angle	1.164
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	PHENIX (1.17.1_3660)
Refinement software	PHENIX ((1.17.1_3660: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.796
High resolution limit [Å]	2.700	2.700
Number of reflections	143455	9096
<I/σ(I)>	9.51
Completeness [%]	97.9
Redundancy	4.7
CC(1/2)	0.992	0.696

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		295	0.65M Na,K tartrate, 0.2M lithium sulfate, 3% (w/v) sucrose, 0.1M CHES (pH 9.5)