7WBK

Crystal structure of Legionella pneumophila effector protein Lpg0081

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 5C (4A)
Synchrotron site	PAL/PLS
Beamline	5C (4A)
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-04-29
Detector	DECTRIS EIGER X 9M
Wavelength(s)	0.9792
Spacegroup name	C 1 2 1
Unit cell lengths	117.131, 93.551, 108.746
Unit cell angles	90.00, 104.55, 90.00

Refinement procedure

Resolution	36.080 - 2.740
R-factor	0.1752
Rwork	0.172
R-free	0.21960
Structure solution method	SAD
RMSD bond length	0.009
RMSD bond angle	1.229
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX (1.17.1-3660)
Refinement software	PHENIX (1.17.1_3660)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	36.080	2.838
High resolution limit [Å]	2.740	2.740
Rmerge	0.097
Rmeas	0.105
Rpim	0.040
Number of reflections	29975	20253
<I/σ(I)>	17.45
Completeness [%]	99.2	91.66
Redundancy	7
CC(1/2)	0.994	0.945

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		295	0.65M Na/K tartrate, 0.2M lithium sulfate, 3% (w/v) sucrose, 0.1M CHES (pH 9.5)