7WBD

Crystal structure of lysozyme (multilcrystal diffraction, CrystFEL/XGANDALF)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 11C
Synchrotron site	PAL/PLS
Beamline	11C
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-06-12
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.9795
Spacegroup name	P 43 21 2
Unit cell lengths	79.510, 79.510, 37.620
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	56.220 - 1.900
R-factor	0.2111
Rwork	0.207
R-free	0.26390
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	7dtb
Data reduction software	CrystFEL
Data scaling software	CrystFEL
Phasing software	MOLREP
Refinement software	PHENIX (1.18.2_3874)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	80.000	1.970
High resolution limit [Å]	1.900	1.900
Number of reflections	10003	966
<I/σ(I)>	4.79	2.58
Completeness [%]	100.0
Redundancy	235.6
CC(1/2)	0.949	0.473

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	SMALL TUBES	4.5	293.15	sodium acetate, PEG 8000, NaCl