7WB3
Crystal structure of T. maritima Rex in ternary complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL44XU |
| Synchrotron site | SPring-8 |
| Beamline | BL44XU |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2021-04-09 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.999994 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 69.140, 62.844, 68.937 |
| Unit cell angles | 90.00, 108.71, 90.00 |
Refinement procedure
| Resolution | 45.342 - 2.401 |
| R-factor | 0.1979 |
| Rwork | 0.195 |
| R-free | 0.25090 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5zz6 |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.442 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.10.1_2155: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.530 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Rmerge | 0.139 | 0.440 |
| Number of reflections | 22008 | 737 |
| <I/σ(I)> | 5.5 | 2.1 |
| Completeness [%] | 99.7 | 98.1 |
| Redundancy | 3.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 296 | Bis-Tris pH 6.5, polypropylene glycol P400 |
