7WAO
Glutamyl-tRNA synthetase from Plasmodium falciparum (PfERS) in complex with Mn
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SOLEIL BEAMLINE PROXIMA 2 |
| Synchrotron site | SOLEIL |
| Beamline | PROXIMA 2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2020-03-15 |
| Detector | DECTRIS EIGER X 9M |
| Wavelength(s) | 0.9840 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 156.750, 46.880, 94.530 |
| Unit cell angles | 90.00, 108.88, 90.00 |
Refinement procedure
| Resolution | 45.329 - 2.582 |
| R-factor | 0.1886 |
| Rwork | 0.186 |
| R-free | 0.24570 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1nyl |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.15rc1_3423) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 45.330 | 2.650 |
| High resolution limit [Å] | 2.580 | 2.580 |
| Rmeas | 0.123 | 1.224 |
| Number of reflections | 20654 | 1370 |
| <I/σ(I)> | 11.1 | 1.3 |
| Completeness [%] | 99.2 | 89.4 |
| Redundancy | 6.8 | 6.6 |
| CC(1/2) | 1.000 | 0.690 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 293 | 0.2 M Lithium sulfate, 0.1 M Tris pH 8.5, 30 % w/v PEG 4000 |
