7WAL

Glutamyl-tRNA synthetase from Plasmodium falciparum (PfERS) in complex with Co

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SOLEIL BEAMLINE PROXIMA 2
Synchrotron site	SOLEIL
Beamline	PROXIMA 2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-03-15
Detector	DECTRIS EIGER X 9M
Wavelength(s)	0.9840
Spacegroup name	C 1 2 1
Unit cell lengths	158.730, 46.110, 95.350
Unit cell angles	90.00, 110.73, 90.00

Refinement procedure

Resolution	46.098 - 2.290
R-factor	0.2108
Rwork	0.208
R-free	0.25550
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	INYL
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX (1.15rc1_3423)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	46.100	2.350
High resolution limit [Å]	2.290	2.290
Rmeas	0.070	1.378
Number of reflections	29435	2036
<I/σ(I)>	14.8	1.1
Completeness [%]	99.7	95
Redundancy	6.8	6.7
CC(1/2)	1.000	0.690

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.5	293	0.2 M Lithium sulfate, 0.1 M Tris pH 8.5, 30 % w/v PEG 4000