7WAJ

Glutamyl-tRNA synthetase from Plasmodium falciparum (PfERS) complexed with ATP and Co

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SOLEIL BEAMLINE PROXIMA 2
Synchrotron site	SOLEIL
Beamline	PROXIMA 2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-03-15
Detector	DECTRIS EIGER X 9M
Wavelength(s)	0.99
Spacegroup name	C 1 2 1
Unit cell lengths	158.190, 46.340, 95.320
Unit cell angles	90.00, 110.72, 90.00

Refinement procedure

Resolution	49.040 - 2.254
R-factor	0.2012
Rwork	0.199
R-free	0.24660
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	7wai
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX (1.15rc1_3423)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	49.040	2.310
High resolution limit [Å]	2.250	2.250
Rmeas	0.061	1.468
Number of reflections	30851	2181
<I/σ(I)>	16.6	1.2
Completeness [%]	99.7	96
Redundancy	6.8
CC(1/2)	1.000	0.740

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.5	293	0.2 M Lithium sulfate, 0.1 M Tris pH 8.5, 30 % w/v PEG 4000