7WA8

Strigolactone receptors in Striga ShHTL7

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U
Synchrotron site	SSRF
Beamline	BL17U
Temperature [K]	100
Detector technology	CCD
Collection date	2017-03-29
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.9792
Spacegroup name	P 21 21 21
Unit cell lengths	74.573, 78.338, 90.923
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	46.437 - 2.330
R-factor	0.2012
Rwork	0.198
R-free	0.26770
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5z8p
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.14_3260)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.370
High resolution limit [Å]	2.330	6.320	2.330
Rmerge	0.110	0.053	0.611
Rmeas	0.119	0.058	0.661
Rpim	0.045	0.023	0.251
Total number of observations	164850
Number of reflections	23355	1292	1143
<I/σ(I)>	10.6
Completeness [%]	100.0	99.8	99.9
Redundancy	7.1	6.2	6.8
CC(1/2)		0.998	0.864

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		290	20% PEG 3350, 4mM calcium chloride, 20mM sodium acetate pH 4.6, 6% (v/v) (+/-)-2-Methyl-2,4-pentanediol, 160mM magnesium chloride, 80mM Tris pH 8.5