7WA4
Crystal structure of GIGANTEA in complex with LKP2
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PAL/PLS BEAMLINE 11C |
| Synchrotron site | PAL/PLS |
| Beamline | 11C |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2021-03-11 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.97944 |
| Spacegroup name | P 61 |
| Unit cell lengths | 104.750, 104.750, 232.161 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 90.720 - 3.500 |
| R-factor | 0.2503 |
| Rwork | 0.245 |
| R-free | 0.29440 |
| Structure solution method | SAD |
| Data reduction software | MOSFLM |
| Data scaling software | Aimless |
| Phasing software | AutoSol |
| Refinement software | PHENIX (1.19rc5_4047) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 100.000 | 3.830 |
| High resolution limit [Å] | 3.500 | 3.500 |
| Rmerge | 0.177 | 1.241 |
| Number of reflections | 18181 | 4311 |
| <I/σ(I)> | 7.7 | |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 9.19 | 9.49 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | MICROBATCH | 6.5 | 293 | 12% MPD, 3%(v/v) PEG 3350, 0.2M ammonium sulfate, 0.1M imidazole/HCl pH 6.5 |
