7W9Z

Crystal structure of Bacillus subtilis YugJ in complex with NADP and nitrate

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 7A (6B, 6C1)
Synchrotron site	PAL/PLS
Beamline	7A (6B, 6C1)
Temperature [K]	100
Detector technology	CCD
Collection date	2015-07-12
Detector	ADSC QUANTUM 270
Wavelength(s)	1.0000
Spacegroup name	P 1 21 1
Unit cell lengths	56.506, 67.694, 102.374
Unit cell angles	90.00, 100.15, 90.00

Refinement procedure

Resolution	28.914 - 1.650
R-factor	0.175
Rwork	0.174
R-free	0.19740
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	7w9x
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.14_3260)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	1.680
High resolution limit [Å]	1.650	1.650
Rmerge	0.077	0.441
Number of reflections	91189	4474
<I/σ(I)>	27.4	4.2
Completeness [%]	99.6	99
Redundancy	3.7	3.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		291	PEG 3350, ammonium nitrate, MES