7W9X

Crystal structure of Bacillus subtilis YugJ in complex with nickel

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 7A (6B, 6C1)
Synchrotron site	PAL/PLS
Beamline	7A (6B, 6C1)
Temperature [K]	100
Detector technology	CCD
Collection date	2015-04-12
Detector	ADSC QUANTUM 270
Wavelength(s)	1.0000
Spacegroup name	P 21 21 21
Unit cell lengths	62.664, 114.522, 118.736
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.288 - 2.151
R-factor	0.2051
Rwork	0.203
R-free	0.24070
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1vlj
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.14_3260)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	2.190
High resolution limit [Å]	2.150	2.150
Rmerge	0.068	0.500
Number of reflections	47100	2297
<I/σ(I)>	32.6	5
Completeness [%]	99.9	100
Redundancy	4.9	5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		291	PEG 6000, Tris