7W9H
Crystal structure of the receiver domain of the transcription regulator FleR from Pseudomonas aeruginosa
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06DA |
| Synchrotron site | SLS |
| Beamline | X06DA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-05-12 |
| Detector | DECTRIS PILATUS 2M-F |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 33.715, 67.712, 51.429 |
| Unit cell angles | 90.00, 92.17, 90.00 |
Refinement procedure
| Resolution | 51.390 - 2.280 |
| R-factor | 0.1881 |
| Rwork | 0.186 |
| R-free | 0.22100 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1qkk |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.005 |
| Data reduction software | DIALS |
| Data scaling software | Aimless |
| Refinement software | PHENIX (1.17.1_3660) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 67.710 | 2.350 |
| High resolution limit [Å] | 2.280 | 2.280 |
| Number of reflections | 10642 | 959 |
| <I/σ(I)> | 7.3 | |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 6.3 | 6 |
| CC(1/2) | 0.983 | 0.840 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 298 | PEG 5000 MME, Calcium Acetate |
