7W9H

Crystal structure of the receiver domain of the transcription regulator FleR from Pseudomonas aeruginosa

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06DA
Synchrotron site	SLS
Beamline	X06DA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-05-12
Detector	DECTRIS PILATUS 2M-F
Wavelength(s)	1.0
Spacegroup name	P 1 21 1
Unit cell lengths	33.715, 67.712, 51.429
Unit cell angles	90.00, 92.17, 90.00

Refinement procedure

Resolution	51.390 - 2.280
R-factor	0.1881
Rwork	0.186
R-free	0.22100
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1qkk
RMSD bond length	0.007
RMSD bond angle	1.005
Data reduction software	DIALS
Data scaling software	Aimless
Refinement software	PHENIX (1.17.1_3660)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	67.710	2.350
High resolution limit [Å]	2.280	2.280
Number of reflections	10642	959
<I/σ(I)>	7.3
Completeness [%]	100.0	100
Redundancy	6.3	6
CC(1/2)	0.983	0.840

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8	298	PEG 5000 MME, Calcium Acetate