7W91

Residues 440-490 of centrosomal protein 63

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 5C (4A)
Synchrotron site	PAL/PLS
Beamline	5C (4A)
Temperature [K]	100
Detector technology	CCD
Collection date	2015-05-29
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.97929
Spacegroup name	P 1
Unit cell lengths	80.622, 81.112, 80.846
Unit cell angles	65.33, 60.07, 60.20

Refinement procedure

Resolution	36.229 - 3.292
R-factor	0.2324
Rwork	0.229
R-free	0.26070
Structure solution method	SAD
RMSD bond length	0.003
RMSD bond angle	0.480
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX (1.10.1_2155)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	3.360
High resolution limit [Å]	3.292	3.292
Rmerge	0.111	0.221
Number of reflections	37129	1383
<I/σ(I)>	17.92
Completeness [%]	90.4
Redundancy	2.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION		291	0.6 M sodium citrate tribasic dihydrate, 0.1 M Tris-HCl pH 7.75, 0.4 M NDSB-221, 0.1M ethylene-diamine-tetraacetic acid