7W8B

Crystal Structure of human Focal Adhesion Targeting (FAT) domain of the Focal Adhesion Kinase

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SOLEIL BEAMLINE PROXIMA 2
Synchrotron site	SOLEIL
Beamline	PROXIMA 2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-11-14
Detector	DECTRIS EIGER X 9M
Wavelength(s)	0.98011
Spacegroup name	C 2 2 21
Unit cell lengths	39.333, 94.311, 85.557
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	47.155 - 2.093
Rwork	0.239
R-free	0.28620
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1ow6
RMSD bond length	0.006
RMSD bond angle	1.540
Data reduction software	XDS (Version Jan 31, 2020)
Data scaling software	Aimless (0.7.4)
Phasing software	MoRDa (1.4.01)
Refinement software	REFMAC (5.8.0267)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	47.160	2.150
High resolution limit [Å]	2.090	2.090
Rmerge	0.165	4.810
Number of reflections	9713	743
<I/σ(I)>	9.7
Completeness [%]	99.5	94.8
Redundancy	13
CC(1/2)	0.998	0.528

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8	293	2500 mM Sodium chloride 100 mM Imidazole/ Hydrochloric acid pH 8.0