7W89
The structure of Deinococcus radiodurans Yqgf
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL02U1 |
| Synchrotron site | SSRF |
| Beamline | BL02U1 |
| Temperature [K] | 80 |
| Detector technology | CCD |
| Collection date | 2021-11-20 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.9792 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 73.070, 49.380, 33.520 |
| Unit cell angles | 90.00, 101.67, 90.00 |
Refinement procedure
| Resolution | 27.069 - 1.500 |
| R-factor | 0.194792841433 |
| Rwork | 0.195 |
| R-free | 0.19557 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1ovq |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.043 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.11.1_2575) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 27.070 | 1.540 |
| High resolution limit [Å] | 1.500 | 1.500 |
| Rmerge | 0.055 | 0.132 |
| Number of reflections | 19580 | 1430 |
| <I/σ(I)> | 26.04 | |
| Completeness [%] | 93.7 | |
| Redundancy | 4.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 289 | 0.1 M MgCl2, 0.1M HEPES (pH 7.0), 15% PEG 4000 |
