7W89

The structure of Deinococcus radiodurans Yqgf

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL02U1
Synchrotron site	SSRF
Beamline	BL02U1
Temperature [K]	80
Detector technology	CCD
Collection date	2021-11-20
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.9792
Spacegroup name	C 1 2 1
Unit cell lengths	73.070, 49.380, 33.520
Unit cell angles	90.00, 101.67, 90.00

Refinement procedure

Resolution	27.069 - 1.500
R-factor	0.194792841433
Rwork	0.195
R-free	0.19557
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1ovq
RMSD bond length	0.010
RMSD bond angle	1.043
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX (1.11.1_2575)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	27.070	1.540
High resolution limit [Å]	1.500	1.500
Rmerge	0.055	0.132
Number of reflections	19580	1430
<I/σ(I)>	26.04
Completeness [%]	93.7
Redundancy	4.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7	289	0.1 M MgCl2, 0.1M HEPES (pH 7.0), 15% PEG 4000