7W7Q

Crystal structure of 1F11S antibody(ScFv)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 5C (4A)
Synchrotron site	PAL/PLS
Beamline	5C (4A)
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-07-11
Detector	DECTRIS EIGER X 9M
Wavelength(s)	0.9796
Spacegroup name	C 1 2 1
Unit cell lengths	79.100, 68.350, 90.710
Unit cell angles	90.00, 108.45, 90.00

Refinement procedure

Resolution	28.680 - 1.960
R-factor	0.2263
Rwork	0.224
R-free	0.27730
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5gs3
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.19.2_4158)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	28.680	2.030
High resolution limit [Å]	1.960	1.960
Number of reflections	32477	3215
<I/σ(I)>	9.87
Completeness [%]	97.2
Redundancy	3.9
CC(1/2)	0.998	0.931

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	EVAPORATION		293	10% PEG 3350, 100 mM Na/K tartrate