7W7H

S Suis FakA-FakB2 complex structure

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U1
Synchrotron site	SSRF
Beamline	BL17U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-06-08
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.9792
Spacegroup name	P 43 21 2
Unit cell lengths	107.749, 107.749, 398.999
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	45.261 - 2.600
R-factor	0.1843
Rwork	0.182
R-free	0.22060
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4x9x
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.14_3260)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	45.261	2.693
High resolution limit [Å]	2.600	2.600
Rmerge	0.079	1.192
Rpim	0.029
Number of reflections	73275	7232
<I/σ(I)>	12.13
Completeness [%]	99.5	99.92
Redundancy	8.7	8.6
CC(1/2)	0.999	0.840

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.5	290	2.4M Ammonium Sulfate