7W7D
Heme exporter HrtBA in complex with heme
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL26B2 |
| Synchrotron site | SPring-8 |
| Beamline | BL26B2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2020-11-25 |
| Detector | RAYONIX MX225HE |
| Wavelength(s) | 1.74000 |
| Spacegroup name | P 1 |
| Unit cell lengths | 82.585, 135.026, 160.969 |
| Unit cell angles | 112.49, 100.41, 93.78 |
Refinement procedure
| Resolution | 49.290 - 3.400 |
| R-factor | 0.2555 |
| Rwork | 0.255 |
| R-free | 0.28480 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6LLL |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.452 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.19.2_4158) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 49.290 | 3.470 |
| High resolution limit [Å] | 3.270 | 3.270 |
| Rmeas | 0.092 | |
| Number of reflections | 187978 | 29851 |
| <I/σ(I)> | 10.6 | |
| Completeness [%] | 97.2 | |
| Redundancy | 4 | |
| CC(1/2) | 0.526 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 277 | 22% PEG 3000, 0.2 M Mg acetate, 0.2 M KCl, 0.1 M HEPES pH 7.0 |
