7W75

Crystal structure of the K. lactis Bre1 RBD in complex with Rad6, crystal form I

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-11-01
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.97853
Spacegroup name	P 65 2 2
Unit cell lengths	94.441, 94.441, 534.644
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	40.890 - 3.200
R-factor	0.2156
Rwork	0.213
R-free	0.26330
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1ayz
Data reduction software	HKL-3000
Data scaling software	SCALEPACK
Phasing software	PHASER
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	3.260
High resolution limit [Å]	3.200	3.200
Number of reflections	24184	1206
<I/σ(I)>	17
Completeness [%]	100.0
Redundancy	18
CC(1/2)	0.985	0.623

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	sodium acetrate, PEG3350