7W6U
Structure of SARS-CoV-2 spike receptor-binding domain complexed with its receptor equine ACE2
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL02U1 |
| Synchrotron site | SSRF |
| Beamline | BL02U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2021-01-30 |
| Detector | DECTRIS EIGER X 9M |
| Wavelength(s) | 0.97918 |
| Spacegroup name | I 41 2 2 |
| Unit cell lengths | 195.570, 195.570, 149.770 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 19.210 - 2.560 |
| R-factor | 0.195 |
| Rwork | 0.193 |
| R-free | 0.22650 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6lzg |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.732 |
| Data reduction software | xia2 |
| Data scaling software | xia2 |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.19rc3_4028) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 31.390 | 2.630 |
| High resolution limit [Å] | 2.560 | 2.560 |
| Rmerge | 0.172 | |
| Rmeas | 0.175 | |
| Rpim | 0.034 | 0.450 |
| Number of reflections | 46770 | 3434 |
| <I/σ(I)> | 17.8 | |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 26.8 | 26.6 |
| CC(1/2) | 0.999 | 0.728 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 277 | 10% (w/v) PEG 1000, 10% (w/v) PEG 8000 |
