7W65
Crystal structure of minor pilin TcpB from Vibrio cholerae complexed with secreted protein TcpF
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL38B1 |
| Synchrotron site | SPring-8 |
| Beamline | BL38B1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-01-30 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 63 2 2 |
| Unit cell lengths | 284.139, 284.139, 297.599 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 31.830 - 4.050 |
| R-factor | 0.2768 |
| Rwork | 0.274 |
| R-free | 0.30080 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 7w63 |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.737 |
| Data reduction software | XDS |
| Data scaling software | Aimless (0.5.32) |
| Phasing software | PHENIX |
| Refinement software | PHENIX (v1.19.2-4092) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 49.210 | 49.210 | 4.160 |
| High resolution limit [Å] | 4.050 | 17.650 | 4.050 |
| Rmerge | 0.170 | 0.050 | 3.072 |
| Rmeas | 0.175 | 0.052 | 3.153 |
| Rpim | 0.040 | 0.014 | 0.707 |
| Total number of observations | 1100587 | 8447 | 87635 |
| Number of reflections | 57983 | 774 | 4439 |
| <I/σ(I)> | 13.1 | 42 | 1.3 |
| Completeness [%] | 99.9 | 94.2 | 100 |
| Redundancy | 19 | 10.9 | 19.7 |
| CC(1/2) | 0.999 | 0.999 | 0.533 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 273 | 0.4 M trisodium citrate, 4%(w/v) PEG3350, 10%(w/v) xylitol. |
