7W64
Crystal structure of minor pilin TcpB from Vibrio cholerae complexed with N-terminal peptide fragment of TcpF
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL38B1 |
| Synchrotron site | SPring-8 |
| Beamline | BL38B1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-01-30 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 74.883, 128.555, 327.242 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 37.200 - 2.300 |
| R-factor | 0.2258 |
| Rwork | 0.222 |
| R-free | 0.26400 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 7w63 |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.072 |
| Data reduction software | XDS |
| Data scaling software | Aimless (0.5.32) |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.19-4092) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 48.770 | 48.770 | 2.340 |
| High resolution limit [Å] | 2.300 | 12.600 | 2.300 |
| Rmerge | 0.084 | 0.038 | 1.069 |
| Rmeas | 0.089 | 0.041 | 1.128 |
| Rpim | 0.029 | 0.014 | 0.351 |
| Total number of observations | 1413980 | 7469 | 71962 |
| Number of reflections | 139539 | 928 | 6930 |
| <I/σ(I)> | 12.1 | 28.3 | 2 |
| Completeness [%] | 99.1 | 94.9 | 99.7 |
| Redundancy | 10.1 | 8 | 10.4 |
| CC(1/2) | 0.997 | 0.998 | 0.851 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 0.1 M HEPES, 0.28 M CaCl2, 22%(v/v) PEG400, pH7.5 |
