7W63

Crystal structure of minor pilin TcpB from Vibrio cholerae

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL38B1
Synchrotron site	SPring-8
Beamline	BL38B1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-12-15
Detector	DECTRIS PILATUS 6M
Wavelength(s)	1.0
Spacegroup name	I 4 2 2
Unit cell lengths	170.830, 170.830, 262.860
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	23.370 - 2.320
R-factor	0.1772
Rwork	0.174
R-free	0.20760
Structure solution method	SAD
RMSD bond length	0.009
RMSD bond angle	1.183
Data reduction software	XDS
Data scaling software	Aimless (0.5.32)
Phasing software	PHASER
Refinement software	PHENIX (v1.19.2-4158)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	47.750	47.750	2.360
High resolution limit [Å]	2.320	12.060	2.320
Rmerge	0.207	0.080	2.271
Rmeas	0.210	0.081	2.299
Rpim	0.034	0.014	0.360
Total number of observations	3267250	19451	183488
Number of reflections	83755	681	4522
<I/σ(I)>	15.3	37	1.9
Completeness [%]	100.0	98.4	100
Redundancy	39	28.6	40.6
CC(1/2)	0.999	0.999	0.856

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	0.1 M Tris-HCl, 1.8 M Lithium Sulfate Monohydrate, pH8.8