7W63
Crystal structure of minor pilin TcpB from Vibrio cholerae
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL38B1 |
| Synchrotron site | SPring-8 |
| Beamline | BL38B1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-12-15 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 1.0 |
| Spacegroup name | I 4 2 2 |
| Unit cell lengths | 170.830, 170.830, 262.860 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 23.370 - 2.320 |
| R-factor | 0.1772 |
| Rwork | 0.174 |
| R-free | 0.20760 |
| Structure solution method | SAD |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.183 |
| Data reduction software | XDS |
| Data scaling software | Aimless (0.5.32) |
| Phasing software | PHASER |
| Refinement software | PHENIX (v1.19.2-4158) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 47.750 | 47.750 | 2.360 |
| High resolution limit [Å] | 2.320 | 12.060 | 2.320 |
| Rmerge | 0.207 | 0.080 | 2.271 |
| Rmeas | 0.210 | 0.081 | 2.299 |
| Rpim | 0.034 | 0.014 | 0.360 |
| Total number of observations | 3267250 | 19451 | 183488 |
| Number of reflections | 83755 | 681 | 4522 |
| <I/σ(I)> | 15.3 | 37 | 1.9 |
| Completeness [%] | 100.0 | 98.4 | 100 |
| Redundancy | 39 | 28.6 | 40.6 |
| CC(1/2) | 0.999 | 0.999 | 0.856 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 0.1 M Tris-HCl, 1.8 M Lithium Sulfate Monohydrate, pH8.8 |
