7W62

Mevo lectin complex with mannotetraose (Man4)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ELETTRA BEAMLINE 11.2C
Synchrotron site	ELETTRA
Beamline	11.2C
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-08-05
Detector	DECTRIS PILATUS 6M
Wavelength(s)	1.0
Spacegroup name	P 21 21 21
Unit cell lengths	96.210, 168.300, 170.249
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	85.270 - 2.000
Rwork	0.226
R-free	0.24960
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	7ded
RMSD bond length	0.013
RMSD bond angle	1.619
Data reduction software	iMOSFLM
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	REFMAC (5.8.0258)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	96.210	2.110
High resolution limit [Å]	2.000	2.000
Rmerge	0.140	1.330
Number of reflections	2231847	186472
<I/σ(I)>	12.1
Completeness [%]	100.0
Redundancy	12
CC(1/2)	0.990	0.680

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	MICROBATCH	6	295	Tacsimate pH 6.0, 0.1 M MES monohydrate pH 6.0, 25% (w/v) polyethylene glycol 4000