7W62
Mevo lectin complex with mannotetraose (Man4)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ELETTRA BEAMLINE 11.2C |
| Synchrotron site | ELETTRA |
| Beamline | 11.2C |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2021-08-05 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 96.210, 168.300, 170.249 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 85.270 - 2.000 |
| Rwork | 0.226 |
| R-free | 0.24960 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 7ded |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.619 |
| Data reduction software | iMOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0258) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 96.210 | 2.110 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.140 | 1.330 |
| Number of reflections | 2231847 | 186472 |
| <I/σ(I)> | 12.1 | |
| Completeness [%] | 100.0 | |
| Redundancy | 12 | |
| CC(1/2) | 0.990 | 0.680 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | MICROBATCH | 6 | 295 | Tacsimate pH 6.0, 0.1 M MES monohydrate pH 6.0, 25% (w/v) polyethylene glycol 4000 |
