7W61

Crystal structure of farnesol dehydrogenase from Helicoverpa armigera

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU MICROMAX-007 HF
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-11-09
Detector	RIGAKU HyPix-6000HE
Wavelength(s)	1.5418
Spacegroup name	I 2 3
Unit cell lengths	118.016, 118.016, 118.016
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.520 - 1.600
R-factor	0.1693
Rwork	0.168
R-free	0.19320
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1xg5
RMSD bond length	0.018
RMSD bond angle	2.334
Data reduction software	CrysalisPro
Data scaling software	Aimless
Phasing software	MOLREP
Refinement software	REFMAC (5.8.0267)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	29.520	1.630
High resolution limit [Å]	1.600	1.600
Rmerge	0.088	0.503
Number of reflections	36084	1742
<I/σ(I)>	24.7	5.6
Completeness [%]	100.0	100
Redundancy	26.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	PEG 4000, sodium acetate