7W5Q

The Kruppel-associated box (KRAB) domain of human zinc finger protein 568

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 7A (6B, 6C1)
Synchrotron site	PAL/PLS
Beamline	7A (6B, 6C1)
Temperature [K]	100
Detector technology	CCD
Collection date	2021-10-28
Detector	ADSC QUANTUM 270
Wavelength(s)	0.979
Spacegroup name	C 1 2 1
Unit cell lengths	84.736, 147.094, 41.891
Unit cell angles	90.00, 90.01, 90.00

Refinement procedure

Resolution	41.890 - 2.730
R-factor	0.2468
Rwork	0.244
R-free	0.31240
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1v65
RMSD bond length	0.012
RMSD bond angle	1.736
Data reduction software	HKL-2000
Data scaling software	CCP4i
Phasing software	PHASER
Refinement software	PHENIX (1.14_3260)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.660
High resolution limit [Å]	2.610	2.610
Number of reflections	13618	576
<I/σ(I)>	31
Completeness [%]	98.2
Redundancy	6.7
CC(1/2)	0.895	0.094

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293.15	PEG 3350, Magnesium formate dihydrate