7W5F
The apo structure of trichobrasilenol synthase TaTC6 with the space group of monoclinic
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | LIQUID ANODE |
| Source details | BRUKER METALJET |
| Temperature [K] | 100 |
| Detector technology | CMOS |
| Collection date | 2021-05-11 |
| Detector | BRUKER PHOTON 100 |
| Wavelength(s) | 1.34138 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 53.984, 97.791, 72.296 |
| Unit cell angles | 90.00, 94.47, 90.00 |
Refinement procedure
| Resolution | 36.190 - 2.530 |
| R-factor | 0.1931 |
| Rwork | 0.188 |
| R-free | 0.25270 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4okm |
| Data reduction software | SAINT |
| Data scaling software | SAINT |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.17.1_3660) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 36.200 | 2.540 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Rmerge | 0.156 | 0.466 |
| Number of reflections | 48370 | 1102 |
| <I/σ(I)> | 6.4 | 1.9 |
| Completeness [%] | 99.5 | |
| Redundancy | 4.3 | |
| CC(1/2) | 0.990 | 0.760 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | EVAPORATION | 298 | 1.2 M Sodium malonate, pH 6.0 |
