7W5D
High resolution structure of lectin-ike Ox-LDL Receptor 1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-07-16 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 0.984 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 71.033, 49.726, 76.580 |
| Unit cell angles | 90.00, 98.57, 90.00 |
Refinement procedure
| Resolution | 16.620 - 1.141 |
| R-factor | 0.1809 |
| Rwork | 0.180 |
| R-free | 0.19310 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1ypq |
| RMSD bond length | 0.009 |
| RMSD bond angle | 0.990 |
| Data reduction software | autoPROC (1.0.5) |
| Data scaling software | autoPROC (1.0.5) |
| Phasing software | PHASER (2.8.3) |
| Refinement software | BUSTER (2.10.4 (16-JUL-2021)) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 75.724 | 75.724 | 1.095 |
| High resolution limit [Å] | 1.010 | 2.970 | 1.010 |
| Rmerge | 0.037 | 0.023 | 0.601 |
| Rmeas | 0.045 | 0.028 | 0.732 |
| Rpim | 0.025 | 0.016 | 0.411 |
| Total number of observations | 307143 | 15305 | 15333 |
| Number of reflections | 105602 | 5280 | 5281 |
| <I/σ(I)> | 12 | 40.1 | 1.6 |
| Completeness [%] | 91.2 | 94.4 | 59.8 |
| Redundancy | 2.9 | 2.9 | 2.9 |
| CC(1/2) | 0.999 | 0.998 | 0.627 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 293.15 | protein 8mg/ml, 10mM Tris-HCl, 150mM NaCl, 1.1 M Sodium Malonate, 0.1M HEPES pH 7, 0.5% v/v Jeffamine ED-2001 |
