7W51
Crystal structure of fragmin domain-1 in complex with actin (ADP-form)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | AichiSR BEAMLINE BL2S1 |
| Synchrotron site | AichiSR |
| Beamline | BL2S1 |
| Temperature [K] | 95 |
| Detector technology | CCD |
| Collection date | 2018-05-30 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 1.12 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 57.220, 91.141, 115.240 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 30.310 - 1.200 |
| R-factor | 0.1519 |
| Rwork | 0.151 |
| R-free | 0.17600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 7w4z |
| Data reduction software | MOSFLM (7.2.2) |
| Data scaling software | SCALA (3.3.22) |
| Phasing software | MOLREP (11.6.02) |
| Refinement software | PHENIX (1.18) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 71.486 | 37.088 | 1.260 |
| High resolution limit [Å] | 1.200 | 3.790 | 1.200 |
| Rmerge | 0.068 | 1.256 | |
| Rmeas | 0.117 | 0.073 | 1.379 |
| Rpim | 0.045 | 0.027 | 0.562 |
| Total number of observations | 1142877 | 43992 | 141418 |
| Number of reflections | 182217 | 6263 | 25464 |
| <I/σ(I)> | 8.1 | 23.4 | 1.2 |
| Completeness [%] | 97.0 | 98.7 | 93.7 |
| Redundancy | 6.3 | 7 | 5.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 0.1 M HEPES-NaOH, pH 8.0, 16% PEG3350 |
