7W4R
Structure of the M. tuberculosis HtrA N269A mutant at room-temperature
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU FR-E+ SUPERBRIGHT |
Temperature [K] | 293 |
Detector technology | IMAGE PLATE |
Collection date | 2020-11-19 |
Detector | RIGAKU RAXIS IV++ |
Wavelength(s) | 1.5418 |
Spacegroup name | H 3 |
Unit cell lengths | 108.500, 108.500, 62.240 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 37.500 - 2.701 |
R-factor | 0.1714 |
Rwork | 0.168 |
R-free | 0.22380 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 6ieo |
RMSD bond length | 0.003 |
RMSD bond angle | 0.552 |
Data reduction software | MOSFLM |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | PHENIX (1.18.2_3874: ???) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 37.500 | 2.797 |
High resolution limit [Å] | 2.701 | 2.701 |
Number of reflections | 7485 | 751 |
<I/σ(I)> | 6 | |
Completeness [%] | 99.8 | |
Redundancy | 2.5 | |
CC(1/2) | 0.967 | 0.263 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | MICROBATCH | 7.5 | 298 | 0.1M HEPES, 1.4M Sodium Citrate tribasic dihydrate pH 7.5 |