7W4B
Phloem lectin (PP2) structure -complex with Chitotrise
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU ULTRAX 18 |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2021-01-19 |
Detector | RIGAKU RAXIS IV++ |
Wavelength(s) | 1.5418 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 83.290, 92.410, 280.780 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 51.665 - 2.500 |
Rwork | 0.287 |
R-free | 0.30170 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 7vwb |
RMSD bond length | 0.009 |
RMSD bond angle | 1.600 |
Data reduction software | iMOSFLM |
Data scaling software | SCALA |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0258) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 53.670 | 2.640 |
High resolution limit [Å] | 2.500 | 2.500 |
Rmerge | 0.320 | 0.790 |
Number of reflections | 560931 | 89566 |
<I/σ(I)> | 5.1 | |
Completeness [%] | 89.3 | |
Redundancy | 8.3 | |
CC(1/2) | 0.960 | 0.880 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | MICROBATCH | 7.2 | 295 | 0.1 M Imidazole pH 7.0, 20% w/v Polyethylene glycol 6,000 |