7W4B

Phloem lectin (PP2) structure -complex with Chitotrise

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU ULTRAX 18
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2021-01-19
Detector	RIGAKU RAXIS IV++
Wavelength(s)	1.5418
Spacegroup name	P 21 21 21
Unit cell lengths	83.290, 92.410, 280.780
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	51.610 - 2.500
R-factor	0.28287
Rwork	0.282
R-free	0.29687
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	7vwb
RMSD bond length	0.008
RMSD bond angle	1.545
Data reduction software	iMOSFLM
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	REFMAC (5.8.0267)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	53.670	2.640
High resolution limit [Å]	2.500	2.500
Rmerge	0.320	0.790
Number of reflections	64194	89566
<I/σ(I)>	5.1
Completeness [%]	89.3
Redundancy	8.3
CC(1/2)	0.960	0.880

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	MICROBATCH	7.2	295	0.1 M Imidazole pH 7.0, 20% w/v Polyethylene glycol 6,000