7W46

Crystal structure of Bacillus subtilis YjoB with ADP

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 11C
Synchrotron site	PAL/PLS
Beamline	11C
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-09-17
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.9793
Spacegroup name	H 3 2
Unit cell lengths	156.259, 156.259, 302.761
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	45.122 - 2.700
R-factor	0.1891
Rwork	0.187
R-free	0.22310
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	7w42
Data reduction software	MOSFLM
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (dev_3051)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.810
High resolution limit [Å]	2.700	2.700
Rmerge	0.120	0.689
Number of reflections	39371	4361
<I/σ(I)>	16.2	3.8
Completeness [%]	100.0
Redundancy	11.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	MICROBATCH	7.5	293	5% (v/v) PEG 400, 2 M ammonium citrate/citric acid, 10 mM TCEP