7W42

Crystal structure of Bacillus subtilis YjoB

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 11C
Synchrotron site	PAL/PLS
Beamline	11C
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-09-17
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.9793
Spacegroup name	H 3 2
Unit cell lengths	155.600, 155.600, 303.081
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	36.302 - 2.619
R-factor	0.2017
Rwork	0.200
R-free	0.23620
Structure solution method	MAD
Data reduction software	MOSFLM
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (dev_3051)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.720
High resolution limit [Å]	2.619	2.620
Rmerge	0.097	0.342
Number of reflections	42649	4419
<I/σ(I)>	16.6	5.8
Completeness [%]	99.8
Redundancy	10.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	MICROBATCH	7.5	293	5% (v/v) PEG 400, 2 M ammonium, citrate/citric acid (pH 7.5), 10 mM TCEP