7W3W

X-ray structure of apo-VmFbpA, a ferric ion-binding protein from Vibrio metschnikovii

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL44XU
Synchrotron site	SPring-8
Beamline	BL44XU
Temperature [K]	277.15
Detector technology	PIXEL
Collection date	2020-11-13
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.899995
Spacegroup name	P 63 2 2
Unit cell lengths	90.792, 90.792, 149.836
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	45.437 - 1.858
Rwork	0.185
R-free	0.20790
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3wae
Data scaling software	XDS
Phasing software	MOLREP
Refinement software	REFMAC (5.8.0267)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	45.437	1.970
High resolution limit [Å]	1.858	1.860
Number of reflections	58147	25021
<I/σ(I)>	15.17
Completeness [%]	99.8
Redundancy	7.85
CC(1/2)	0.999	0.999

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	7	293.15	0.25M ammonium tartrate dibasic, 25% PEG 3350, 100mM Tris-HCl (pH 7.0)
1	VAPOR DIFFUSION	7	293.15	0.25M ammonium tartrate dibasic, 25% PEG 3350, 100mM Tris-HCl (pH 7.0)