7W3S
The complex structure of Larg1-ADPr from Legionella pneumophila
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U1 |
| Synchrotron site | SSRF |
| Beamline | BL17U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2020-12-18 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.97918 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 109.250, 92.812, 116.053 |
| Unit cell angles | 90.00, 105.33, 90.00 |
Refinement procedure
| Resolution | 53.416 - 2.324 |
| R-factor | 0.188 |
| Rwork | 0.186 |
| R-free | 0.22700 |
| Structure solution method | SAD |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.054 |
| Data reduction software | HKL-3000 |
| Data scaling software | xia2 |
| Phasing software | HKL-3000 |
| Refinement software | PHENIX (1.11.1_2575) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 53.420 | 2.407 |
| High resolution limit [Å] | 2.320 | 2.324 |
| Number of reflections | 94202 | 9251 |
| <I/σ(I)> | 12.09 | |
| Completeness [%] | 97.5 | |
| Redundancy | 2 | |
| CC(1/2) | 0.998 | 0.773 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | COUNTER-DIFFUSION | 289.15 | 0.2M Lithium sulfate, 0.1M Tris, pH 7.5, 5% polyethylene glycol 4,000 |
