7W36

Crystal structure of human Atg5 complexed with a stapled peptide

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL32XU
Synchrotron site	SPring-8
Beamline	BL32XU
Temperature [K]	95
Detector technology	PIXEL
Collection date	2020-02-05
Detector	DECTRIS EIGER X 9M
Wavelength(s)	1.0000
Spacegroup name	P 21 21 21
Unit cell lengths	43.794, 73.083, 102.127
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	40.249 - 3.000
R-factor	0.2494
Rwork	0.245
R-free	0.28660
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4tq1
RMSD bond length	0.004
RMSD bond angle	0.792
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHENIX
Refinement software	PHENIX ((1.13_2998: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	40.249	3.030
High resolution limit [Å]	2.870	2.870
Rmeas	0.140
Number of reflections	14426	2288
<I/σ(I)>	7.33
Completeness [%]	99.5
Redundancy	3.6
CC(1/2)		0.638

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	20% PEG 3350, 0.2M MgCl2, 0.1M Tris-HCl pH8.5