7W30
Tudor domain of SMN in complex with a small molecule
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU FR-E |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2017-05-30 |
| Detector | RIGAKU SATURN A200 |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 70.725, 70.725, 119.931 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 35.000 - 1.800 |
| R-factor | 0.2118 |
| Rwork | 0.210 |
| R-free | 0.25790 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4qq6 |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.920 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0257) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 35.360 | 1.840 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Number of reflections | 32704 | 1857 |
| <I/σ(I)> | 29.4 | |
| Completeness [%] | 99.4 | |
| Redundancy | 10.5 | |
| CC(1/2) | 1.000 | 0.774 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 291 | 2 M ammonium sulfate, 0.2 M sodium chloride, 0.1 M Hepes, pH 7.5 |
