7W26

monolignol ferulate transferase

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-04-15
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.979
Spacegroup name	P 41 21 2
Unit cell lengths	136.420, 136.420, 102.890
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	96.460 - 2.430
R-factor	0.2184
Rwork	0.216
R-free	0.26580
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2bgh
Data scaling software	SCALEPACK
Phasing software	PHASER
Refinement software	PHENIX (1.17.1_3660)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	96.460	2.580
High resolution limit [Å]	2.430	2.430
Number of reflections	37114	5867
<I/σ(I)>	13.25
Completeness [%]	99.7
Redundancy	6.78
CC(1/2)	0.998	0.512

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		291	potassium bromide, Tris, PEG400