7W1D
Crystal structure of Klebsiella pneumoniae K1 capsule-specific polysaccharide lyase in a C2 crystal form
This is a non-PDB format compatible entry.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL44XU |
| Synchrotron site | SPring-8 |
| Beamline | BL44XU |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-07-15 |
| Detector | BRUKER SMART 6500 |
| Wavelength(s) | 1.0, 1.05372, 1.07194 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 363.945, 239.626, 121.632 |
| Unit cell angles | 90.00, 100.13, 90.00 |
Refinement procedure
| Resolution | 29.950 - 2.780 |
| R-factor | 0.184 |
| Rwork | 0.182 |
| R-free | 0.21900 |
| Structure solution method | MAD |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX (Phenix1.17.1-3660) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.880 |
| High resolution limit [Å] | 2.780 | 2.780 |
| Rmerge | 0.139 | 0.625 |
| Number of reflections | 257393 | 25697 |
| <I/σ(I)> | 11.24 | 2.47 |
| Completeness [%] | 99.7 | 99.9 |
| Redundancy | 3.2 | 3.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.5 | 293 | 15% (W/V) PEG 6000, 0.1 M CITRATE (PH 5.5), 0.019 M N-DECYL-N,N-DIMETHYLGLYCINE, VAPOR DIFFUSION |
