7W11

glycosyltransferase

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-05-26
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.97915
Spacegroup name	P 1 21 1
Unit cell lengths	50.753, 76.533, 63.210
Unit cell angles	90.00, 97.19, 90.00

Refinement procedure

Resolution	19.790 - 2.450
R-factor	0.1955
Rwork	0.193
R-free	0.24420
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6l8z
RMSD bond length	0.008
RMSD bond angle	0.953
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHENIX
Refinement software	PHENIX (1.19.2_4158)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	19.790	2.550
High resolution limit [Å]	2.450	2.450
Number of reflections	17505	1953
<I/σ(I)>	6.7
Completeness [%]	98.7
Redundancy	6.6
CC(1/2)	0.960	0.728

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		291.15	PEG 3350, 0.1M sodium chloride, 0.1M MES (pH 6.5)