7W0T

TRIM7 in complex with C-terminal peptide of 2C

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL18U1
Synchrotron site	SSRF
Beamline	BL18U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-10-06
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.979
Spacegroup name	P 1 21 1
Unit cell lengths	79.361, 53.758, 81.299
Unit cell angles	90.00, 119.00, 90.00

Refinement procedure

Resolution	42.450 - 1.570
R-factor	0.1693
Rwork	0.168
R-free	0.18640
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6uma
RMSD bond length	0.008
RMSD bond angle	1.009
Data reduction software	autoPROC
Data scaling software	autoPROC
Phasing software	PHASER
Refinement software	PHENIX (1.19rc6_4061)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	42.538	1.625
High resolution limit [Å]	1.569	1.569
Number of reflections	83192	8288
<I/σ(I)>	14.33
Completeness [%]	99.0
Redundancy	6.7
CC(1/2)	0.999	0.776

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	LIQUID DIFFUSION		291.15	ammonium acetate, magnesium acetate tetrahydrate, HEPES sodium, polyethylene glycol