7W0Q

TRIM7 in complex with C-terminal peptide of 2C

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL18U1
Synchrotron site	SSRF
Beamline	BL18U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-10-06
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.979
Spacegroup name	C 1 2 1
Unit cell lengths	70.486, 53.178, 46.744
Unit cell angles	90.00, 91.44, 90.00

Refinement procedure

Resolution	46.730 - 1.100
R-factor	0.187
Rwork	0.186
R-free	0.21120
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6uma
RMSD bond length	0.008
RMSD bond angle	1.185
Data reduction software	autoPROC
Data scaling software	autoPROC
Phasing software	PHASER
Refinement software	PHENIX (1.19rc6_4061)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	46.730	1.139
High resolution limit [Å]	1.100	1.100
Number of reflections	60806	3023
<I/σ(I)>	17.69
Completeness [%]	86.7
Redundancy	6.1
CC(1/2)	0.998	0.974

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	LIQUID DIFFUSION		291.15	PEG 3350, potassium nitrate