7W0K

plant glycosyltransferase

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-05-26
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.97915
Spacegroup name	P 1 21 1
Unit cell lengths	54.462, 74.038, 61.815
Unit cell angles	90.00, 114.40, 90.00

Refinement procedure

Resolution	48.440 - 2.040
R-factor	0.1956
Rwork	0.193
R-free	0.24430
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6l8z
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHENIX
Refinement software	PHENIX (1.19.2_4158)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.440	2.100
High resolution limit [Å]	2.040	2.040
Rmerge	0.177
Number of reflections	28193	2165
<I/σ(I)>	8
Completeness [%]	97.4
Redundancy	6.7
CC(1/2)	0.992	0.791

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		291.15	PEG 3350, sodium chloride, MES