7W0J

Acyl-CoA dehydrogenase, Tfu_1647

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17B1
Synchrotron site	SSRF
Beamline	BL17B1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-07-11
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.9793
Spacegroup name	P 1
Unit cell lengths	85.124, 88.908, 97.178
Unit cell angles	90.19, 91.93, 112.09

Refinement procedure

Resolution	27.460 - 3.130
R-factor	0.2174
Rwork	0.215
R-free	0.27040
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3pfd
Data reduction software	HKL-2000
Data scaling software	HKL-3000
Phasing software	HKL-3000
Refinement software	PHENIX (1.19_4092)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	3.200
High resolution limit [Å]	3.100	3.100
Number of reflections	43185	43185
<I/σ(I)>	8
Completeness [%]	95.8
Redundancy	3.6
CC(1/2)	0.931	0.931

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	9	289.15	0.1M Bis-Tis pH 9.0, 30% w/v PEG 6000